Superresolution Microscopy

Stream Leader: Antoine van Oijen

Invited Speakers

Srishti Dar - Tata Institute of Fundamental Research, Mumbai, India. Breaking–down Complexity: in vitro Reconstitution of Cytokinesis.

My graduate research with Dr. Thomas Pucadyil, at Indian Institute of Science Education and Research, Pune has led to the development of a novel assay system of supported membrane tubes that allows for quantitative fluorescence microscopy- based detection and analysis of protein and membrane dynamics (Dar et al., Nature Protocols, 2017). We have used this assay to tease apart the mechanism by which dynamin catalyzes membrane fission (Dar et al., Nature Cell Biology, 2015) and investigate the role of the membrane binding pleckstrin homology domain in the fission reaction (Dar and Pucadyil, MBoC, 2017). Presently, I am working as an INSPIRE faculty at the Tata Institute of Fundamental Research, Mumbai, India on an independent project on building an assay system of protein-encapsulated GUVs to understand actomyosin ring dynamics.

Olga Shimoni - Faculty of Science, University of Technology, Sydney. Intracellular trafficking of single fluorescent nanoparticles.

Dr Olga Shimoni is Senior Lecturer at the UTS Faculty of Science and NHMRC-ARC Dementia Research Fellow. She is also co-CI on the ARC Industrial Research Hub for Integrated Device for End-user Analysis at Low-levels (ARC IDEAL Hub). She graduated with PhD from Melbourne University in Chemical and Biomolecular Engineering in 2012. Her research brings together breakthroughs in physics, chemistry, nanotechnology and biology to improve human health. It spans across creating nanoscale biosensors for earlier detection of Alzheimer's disease; point-of-care tests for celiac disease, tuberculosis or prostate cancer; drug delivery carriers to target diseased tissue; and multi-functional luminescent probes that can monitor organelle activity and interactions. As an earlier career researcher, she received more than $5 million in external grants, published more than 25 research papers in high-impact journals and two patents.

Yujie Sun - School of Life Sciences, Peking University, Beijing 100871, China. Super-resolution study of nuclear structure and dynamics.

Dr. Yujie Sun obtained his Bachelor and Master degrees in Chemistry from the University of Science and Technology of China in 1996 and 1999, respectively. He came to the University of Pittsburgh to pursue his PhD degree. Advised by Professor Gilbert C. Walker, Dr. Sun focused on studies about mechanical properties of biopolymer and synthetic polymeric materials at macroscale and nanoscale using Atomic Force Microscopy. After achieving his PhD, Dr. Sun joined in Dr. Yale E. Goldman's group at the University of Pennsylvania School of Medicine. As a member of the Pennsylvania Muscle Institute and Nano/Bio Interface Center, Dr. Sun worked with an inter-disciplinary team to solve the puzzle about how molecular motor works using single molecule fluorescence and manipulation techniques. In 2011, Dr. Sun joined in the Biodynamic Optical Imaging Center (BIOPIC), Peking University, as an assistant professor. At BIOPIC, Dr. Sun has been developing advanced single molecule imaging and manipulation techniques to study gene regulation in the cell.

Keng-hui Lin, Institute of Physics, Academia Sinica, Taipei, Taiwan. Investigating apical constriction force of Madin-Darby Canine Kidney Cells by laser ablations.

Dr. Lin’s lab focuses on understanding the influence of dimensionality on mammalian cellular behaviors from the view point of mechanobiology. Her lab has developed novel 3D culture scaffolds as monodisperse open solid foam and utilized quantitative image analysis to analyze cell morphology in 3D. Her lab’s latest research project is to investigate the apical tensions of Madin-Darby Canine Kidney cells by laser ablation and image analysis on cell shapes.

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