20-21 November 2014
National Centre for Synchrotron Science
Australia/Melbourne timezone
Save the date: User Meeting 2015 - 26-27 November

LCP crystallisation and its challenge with the D2L receptor

20 Nov 2014, 17:30
1h 30m
NCSS Exhibition Area ()

NCSS Exhibition Area

Australian Synchrotron 800 Blackburn Road Clayton VIC 3168
Board: 901


Dr Connie Darmanin (La Trobe)


Self-assembly lipids have been used to solve a number of G-protein coupled receptor structures to date and the mechanism behind it still remains a mystery. Here we report on two factors; lipid incubation time and protein concentration and investigate three different lipid systems; monoolein (MO), phytantriol (PT) and phytanoyl ethanomide (PE), which influenced the uptake of the Dopamine 2 long (D2L) receptor into the lipid structure. We show reasons why the D2L receptor has proven to be difficult to crystallize in the commercial cubic phase lipid, MO, and show why we need to develop novel crystallization media.
Keywords or phrases (comma separated) GPCR, LCP crystallization, lipids, D2 receptor

Primary author

Dr Connie Darmanin (La Trobe)


Presentation Materials

There are no materials yet.
Your browser is out of date!

Update your browser to view this website correctly. Update my browser now