Speaker
Ms
Hannah Coughlan
(La Trobe University)
Description
For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time using beam line 34-ID-C at the Advanced Photon Source. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.
Primary author
Ms
Hannah Coughlan
(La Trobe University)
Co-authors
Prof.
Brian Abbey
(LTU/ImagingCOE)
Dr
Connie Darmanin
(La Trobe)
Dr
Grant van Riessen
(La Trobe University)
Mr
Nicholas Phillips
(LTU/CXS)
Dr
Ross Harder
(Argonne National Laboratory)
