25-27 November 2015
National Centre for Synchrotron Science
Australia/Melbourne timezone

Low-volume electrochemical cell for in-situ measurement of XAS spectra of reactive metal compounds and biomolecules

26 Nov 2015, 13:30
Exhibition space (National Centre for Synchrotron Science)

Exhibition space

National Centre for Synchrotron Science

Australian Synchrotron 800 Blackburn Road Clayton VIC 3168
Board: BT-33
Poster Beamlines, Instrumentation and Techniques Poster Session 1


Dr Stephen Best (University of Melbourne)


The design and operation of a low volume spectroelectrochemical (SEC) cell for the measurement of X-ray absorption spectra (XAS) of solutions at room temperature will be described. The XAS-SEC cell is suitable for operation with fluorescence detection where the 2×2×12 mm working electrode chamber doubles as the solution cell for XAS measurements. A key element of the design of the experiment is the control of the flow properties of the solution under electrochemical/XAS interrogation where a continuous pulsing of the solution with a small nett flow rate ensures both rapid electrosynthesis and that photoreduction can be controlled. The operation of the XAS-SEC cell is demonstrated by the X-ray absorption near edge spectra (XANES) of solutions of K3[Fe(C2O4)3] under static and dynamic flow conditions and the XANES of [Fe(C2O4)3]4- obtained by electrochemical reduction. The suitability of the approach for the study of protein samples is demonstrated by preliminary measurement of the ubiquitous electron transport protein cytochrome c.
Keywords XAS; XANES; Electrochemistry; Metalloproteins; iron complexes

Primary author

Dr Stephen Best (University of Melbourne)


Dr Aviva Levina (Sydney University) Prof. Peter Lay (Sydney University)

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