Speaker
Ms
Josie Nunn
(Flinders University SA)
Description
This presentation gives an overview of the development and comparison of different sample preparation techniques for presenting samples to the Far-IR and IR beam line at the Australian Synchrotron.
We have investigated using polyethylene (PE), polytetrafluoroethylene (PTFE), polyvinylacetate (PVA), paraffin, and mixtures, as matrixes for pellets.
We have also trialled pressing pure compounds without an added matrix. Most recently we have investigated using specially constructed liquid cells for liquid samples and those compounds that can be in solution.
Not surprisingly, there is not one 'solution' to all sample preparations and there are compromises to be made. Samples that do not bind into stable pellets as pure compounds need a matrix to support them and compounds that are strong absorbers may need matrix to dilute them.
For solutions the choice of solvent is also critical (especially for compounds that are poorly soluble in convenient solvents) as there needs to be enough material to give a signal without being swamped by the signal form the solvent.
Presenting results from those samples that produced good spectra from a range of applications - riboflavin (fluorescence of semen), ninhydrin (from fingerprint developing reagents) and bilirubin and biliverdin (from studying breakdown of blood and ageing of bruises) and those that didn't - this presentation draws conclusions about the process of optimising the preparation of varied samples to obtain the best results from your time at the Australian Synchrotron.
| Keywords or phrases (comma separated) | Far-IR, IR, Pellets, Liquid cells, Semen, Fingerprints, blood, bruises |
|---|---|
| What is your gender? | Female |
| Are you an ECR? (<5 yrs</br>since PhD/Masters) | No |
| Are you a student? | Yes |
| Do you wish to take part in</br>the Student Poster Slam? | No |
Primary author
Ms
Josie Nunn
(Flinders University SA)
Co-authors
Ms
Claire Sully
(Chemical and Physical Sciences, Flinders University, Adelaide)
Danielle Martin
(Australian Synchrotron)
Dr
Dominique Appadoo
(The Australian Synchrotron)
Prof.
Hilton Kobus
(Chemical and Physical Sciences, Flinders University, Adelaide)
Prof.
Neil Langlois
(Forensic Science South Australia)
Ms
Rachel West
(Chemical and Physical Sciences, Flinders University, Adelaide)
Ruth Plathe
(Australian Synchrotron)
Prof.
Stewart Walker
(School of Chemical and Physical Sciences, Flinders University)
Mr
Thomas Hensel
(Chemical and Physical Sciences, Flinders University, Adelaide)
