- Nathan Cowieson ()
4. Mechanisms of action of a potent DNA binding UVA photosensitiser using mRNA-sequencing and infrared Synchrotron microspectroscopy
Dr Karagiannis Tom (Baker IDI Heart and Diabetes Institute)
Phototherapy is a well-established therapeutic strategy in dermatology, particularly for the treatment of psoriasis and cutaneous T-cell lymphoma. Treatment may either rely on the cytotoxic effect of light of a particular wavelength (e.g. UVB and narrowband UVB) or may require the use of a sensitizer (e.g. psoralens with UVA). We have developed iodinated DNA minor groove binding...
133. Correlative single cell Fourier transform infrared spectroscopy and super-resolution fluorescence microscopy reveals the effects of fixation on the biochemistry of mammalian cells.
Ms Donna Whelan (Monash University)
Synchrotron Fourier transform infrared (SFTIR) spectroscopy provides the best available signal-to-noise which allows for fast, sensitive detection of the holistic biochemistry of single live cells. Recently developed super-resolution fluorescence microscopy (SRFM) techniques based on single molecule emissions can yield an order of magnitude improvement in spatial resolution in the imaging of...
Prof. Anthony White (University of Melbourne)
A hallmark of neurodegeneration is a failure of homeostatic mechanisms controlling the concentration and distribution of biometals. A major roadblock to understanding the impact of altered biometal homeostasis in neurodegenerative disease is the lack of specific and sensitive techniques capable of providing quantitative subcellular information on biometals in situ. Advances in X-ray...
Dr Mark Tobin (Australian Synchrotron)
Developments to enable infrared microspectroscopy to extend beyond the far field diffraction limit are being undertaken at several accelerator facilities worldwide. These include the CLIO Free Electron Laser (Paris, France), LNLS (Campinas, Brazil), and the ALS infrared beamline (Berkeley, USA). Without such developments, the spatial resolution in the mid-IR is typically 3 to 5 microns. Two...