20-21 November 2014
National Centre for Synchrotron Science
Australia/Melbourne timezone
Save the date: User Meeting 2015 - 26-27 November

Characterisation of model protein denaturation by SAXS

20 Nov 2014, 17:30
1h 30m
NCSS Exhibition Area ()

NCSS Exhibition Area

Australian Synchrotron 800 Blackburn Road Clayton VIC 3168
Board: 1012


Gloria Xun (Australian Synchrotron)


The biological functions of proteins depend on the ability of the proteins to fold correctly. Misfolding/unfolding of proteins can cause formation of insoluble pathological aggregates, leading to degenerative diseases, such as Alzheimer’s and Parkinson’s [1]. Although considerable studies have been carried out on the molecular mechanism of protein aggregation, the lack of detailed information that links the initial stage of aggregation and the final structure limits the understanding of protein aggregation. In this study, our objective is to investigate the thermal denaturation and pre-aggregation of three soluble model proteins, ribonuclease A, myoglobin and chymotrypsinogen A, with different secondary structure characteristics. By using in situ small angle X-ray scattering, we can extract thermodynamic parameters and study the very early stages of the aggregation process-especially the conformational changes of individual protein molecules in solution on thermal denaturation. Our hypothesis is that the differences in aggregation mechanisms observed in the models proteins during thermal denaturation are intimately related to their secondary structures. These results will be complemented with chemical and high pressure denaturation to gain a complete thermodynamic picture of the different protein-types resistance to aggregation. 1. Tan, S. Y., & Pepys, M. B. (1994). Amyloidosis. Histopathology, 25 (5), 403-414.
Keywords or phrases (comma separated) Protein aggregation, thermal denaturation

Primary author

Gloria Xun (Australian Synchrotron)


Dr Duncan McGillivray (The University of Auckland) Dr Nathan Cowieson (Australian Synchrotron) Dr Nigel Kirby (Australian Synchrotron)

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