20-21 November 2014
National Centre for Synchrotron Science
Australia/Melbourne timezone
Save the date: User Meeting 2015 - 26-27 November

DNA Binding Studies using Synchrotron Radiation Circular Dichroism (SRCD) data and Mathematica

20 Nov 2014, 17:30
1h 30m
NCSS Exhibition Area ()

NCSS Exhibition Area

Australian Synchrotron 800 Blackburn Road Clayton VIC 3168
Board: 301


Prof. Janice Aldrich-Wright (University of Western Sydney)


The binding affinity of a series of square planar achiral platinum(II) compounds of the type [Pt(AL)(IL)]2+, where AL is 1,2-diaminoethane and IL are 1,10-phenanthroline (phen), 4-methyl-1,10-phenanthroline (4-Mephen), 5-methyl-1,10-phenanthroline (5-Mephen), 4,7-dimethyl-1,10-phenanthroline (47-Me2phen), 5,6-dimethyl-1,10-phenanthroline (56-Me2phen) or 3,4,7,8-tetramethyl-1,10-phenanthroline (3478-Me4phen) has been reinvestigated using Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy. SRCD spectroscopy uses the intense light of a synchrotron beam to measure shorter wavelength data, exposing CD bands not previously observed. The additional peaks were considerably more intense than those observed by conventional CD spectroscopy, and could be used for binding affinity determinations. In addition, the authors have reviewed the various mathematical approaches used to estimate equilibrium binding constants and thereby demonstrate that their mathematical approach, which has been implemented with Wolfram Mathematica, has merit over other methods.
Keywords or phrases (comma separated) Synchrotron Radiation Circular Dichroism (SRCD), platinum(II)

Primary author

Prof. Janice Aldrich-Wright (University of Western Sydney)


Dr Bahman Ghadirian Ghadirian (University of Western Sydney) Mr Dale Ang (University of Western Sydney) Prof. Frank Stootman (University of Western Sydney) Dr Nykola Jones (Department of Physics and Astronomy - Institute for Storage Ring Facilities, Aarhus University, Denmark)

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