Speaker
Description
In 2012, Mixed lineage kinase domain-like (MLKL), a catalytically-dead (“zombie”) cousin of conventional protein kinases, termed a pseudokinase, was implicated as the key effector in the programmed necrosis (or necroptosis) cell death pathway. This pathway has been implicated in innate immunity, the pathogenesis of inflammatory diseases, and tissue injury arising from ischemia-reperfusion. As a result, an improved fundamental knowledge of MLKL’s activation mechanism is of enormous interest as we and others look to target the pathway therapeutically.
Here, I will describe our recent work dissecting the chronology of events in this pathway using novel tools, biochemistry, microscopy, proteomics and structural biology methods. Our structural studies were enabled by the MX and SAXS beamlines at the Australian Synchrotron and isotopic protein labelling at the National Deuteration Facility.
| Level of Expertise | Expert |
|---|---|
| Presenter Gender | Man |
| Which facility did you use for your research | Australian Synchrotron |
| Pronouns | He/Him |
| Condition of submission | Yes |
