Speaker
Description
The National Deuteration Facility (NDF) at the Australian Nuclear Science and Technology Organisation (ANSTO) provides deuteration for a diversity of molecules and applications, including the deuteration of proteins for solvent contrast variation of multi-subunit biological assemblies.
Many of the macromolecular functions of life and disease involve dynamic protein interactions. Observing, and mapping, large scale domain movements is a strength of method of solution scattering. Small angle x-ray scattering can quickly provide macromolecular envelope information. Extending on this is the method of small angle neutron scattering (SANS) using solvent contrast variation, and subunit-specific labelling by deuteration, which enables identification and mapping of relative dispositions of the subunits within the macromolecular envelope.
Recent illustrative examples of structural characterisations using deuteration and SANS include: [1] the trimeric Proteus mirabilis ScsC protein bound to ScsB; [2] the BAMLET complex of alpha-lactalbumin and oleic acid; and [3] multiple complexes of syntaxin and munc18.
[1] Disulfide isomerase activity of the dynamic, trimeric Proteus mirabilis ScsC protein is primed by the tandem immunoglobulin-fold domain of ScsB.
Furlong, E. J., Choudhury, H. G., Kurth, F., Duff, A. P., Whitten, A. E. & Martin, J. L.
Journal of Biological Chemistry, 293, 5793-5805. (2018) http://dx.doi.org/10.1074/jbc.RA118.001860
[2] Neutron scattering shows a droplet of oleic acid at the center of the BAMLET complex.
Rath, E. M., Duff, A. P., Gilbert, E. P., Doherty, G., Knott, R. B. & Church, W. B.
Proteins, 85, 1371-1378. (2017) http://dx.doi.org/10.1002/prot.25298
[3] Studying Munc18:syntaxin interactions using small-angle scattering.
Whitten, A. E., Jarrott, R. J., Hu, S. H., Duff, A. P., King, G. J., Martin, J. L. & Christie, M. P.
Methods in Molecular Biology, 1860, 115-144. (2019). http://dx.doi.org/10.1007/978-1-4939-8760-3_7
| Do yo wish to take part in the poster slam | No |
|---|---|
| Travel Funding | No |
| Level of Expertise | Expert |
| Speakers Gender | Male |
