20-21 November 2014
National Centre for Synchrotron Science
Australia/Melbourne timezone
Save the date: User Meeting 2015 - 26-27 November

The effect of increasing radiation doses on normal & malignant cell migration

20 Nov 2014, 17:30
1h 30m
NCSS Exhibition Area ()

NCSS Exhibition Area

Australian Synchrotron 800 Blackburn Road Clayton VIC 3168
Board: 802
Poster Radiotherapy and Radiobiology Welcome Function, Poster Session, Exhibition


Jeff Crosbie (University of Melbourne)


The aim of the project is to investigate how normal and malignant cell migration is influenced by conventional radiotherapy doses as well as by experimental treatments such as microbeam radiation therapy (MRT). Radiation-induced tumor cell migration is a recognized phenomenon that can occur when cells are sub-lethally irradiated. Our group previously demonstrated that tumor cells showed extensive migration 24 hours post-MRT. We irradiated well chamber slides containing cultured normal and malignant cells with a range of doses (2, 5, 10Gy) using a conventional, Cobalt- 60 source. We used time-lapse microscopy (live cell imaging) and image processing techniques to track fluorescently-labelled cells for up to 24 hrs post-RT. Statistical analysis of the live cell microscopy data showed there was a notable change in the vector displacement when cells were irradiated with increasing doses of conventional radiation. The observed changes in cell movement may be due to asynchronous cell cycling and proliferation, subsequently dampened with increasing dosages of radiation. We noted a bi-phasic response in some cases with the initial ‘hit’ of radiation generating an immediate response which switches over to a longer-term response when the damage finally takes its toll on the cells and they slow down to die. The cells’ migratory capacity was clearly affected or modulated by the conventional radiation doses. This modulation could be important for MRT studies since the low, ‘valley’ dose may leave cells sub-lethally irradiated.
Keywords or phrases (comma separated) microbeam radiation therapy, cell migration

Primary author

Jeff Crosbie (University of Melbourne)


Dr Camden Lo (Monash Microscopy Institute) Mr Cameron Nowell (Monash University Parkville) Dr Elizabeth Kyriakou (University of Melbourne) Mr Mohammed Ibahim (University of Melbourne) Prof. Peter Rogers (University of Melbourne) Dr Yuqing Yang (University of Melbourne)

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