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SUMMARY:Synchrotron broad beam and MRT radiation induces DNA damage in nor
 mal mouse tissues distant from the irradiated volume
DTSTART;VALUE=DATE-TIME:20141121T031500Z
DTEND;VALUE=DATE-TIME:20141121T033500Z
DTSTAMP;VALUE=DATE-TIME:20260809T080925Z
UID:indico-contribution-748@events01.synchrotron.org.au
DESCRIPTION:Speakers: Jessica Ventura (Molecular Radiation Biology Laborat
 ory\, Peter MacCallum Cancer Centre\, Melbourne\, VIC\, Australia)\nMicrob
 eam radiation therapy (MRT) is a novel\, preclinical modality\, with a uni
 que ability to generate less radiation damage to neighbouring normal tissu
 es\, while providing efficient ablation to the tumour mass\; compared to t
 he currently used Broad Beam (BB) modality. A comprehensive investigation 
 on the mechanisms and side effects of these modalities have not currently 
 been established. Here we compared the radiation-induced bystander effect 
 (RIBE) of BB and MRT irradiation\, generated by the Imaging and Medical Be
 amline at the Australian Synchrotron in C57BL/6 mice. Animals were irradia
 ted with 10Gy or 40Gy peak dose of BB or MRT\, in an 8x8\, 8x1 and 2x2mm a
 rea on the right hind leg\, using an X-ray beam with a dose rate of 49Gy/s
 ec and constant current of 200mA.  At 24 and 96hrs post-irradiation\, we c
 ollected irradiated skin and an assortment of unirradiated tissue\; these 
 were processed for DSB detection\, using the γH2AX assay. For both modali
 ties the levels of γH2AX foci in unirradiated tissues of irradiated mice\
 , varied in comparison to irradiated animals.  Overall\, MRT and BB induce
 d an elevated γH2AX response at 10Gy\, while inhibiting this response at 
 40Gy. Oxidative clustered DNA lesions (OCDL) in tissues were measured usin
 g constant field gel electrophoresis\, where genomic DNA was treated with 
 purine\, pyrimidine and abasic site-specific enzymes. Results show a marke
 d increase of OCDLs in a variety of unirradiated tissues. We will discuss 
 the role of irradiated volume\, dose\, and beam modality in the manifestat
 ion of the in-vivo RIBE.\n\nhttps://events01.synchrotron.org.au/event/3/co
 ntributions/748/
LOCATION: Conference Room AS
URL:https://events01.synchrotron.org.au/event/3/contributions/748/
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