20-21 November 2014
National Centre for Synchrotron Science
Australia/Melbourne timezone
Save the date: User Meeting 2015 - 26-27 November

Reconciling Synchrotron SAXS Data with NMR Data for a Two-Domain Protein

20 Nov 2014, 17:30
1h 30m
NCSS Exhibition Area ()

NCSS Exhibition Area

Australian Synchrotron 800 Blackburn Road Clayton VIC 3168
Board: 1013


Prof. Geoffrey Jameson (Massey University)


A preliminary structure of the protein Yih1 (Yeast impact homologue 1), a protein of partially characterised function, has been obtained by multi-dimensional NMR methods. The ~300-residue protein has two distinct domains of approximately 120 and 160 residues with an approximately 20-residue linker. However, no NOEs could be found involving contacts between the two domains. Solution-state SAXS data were recorded at the Australian Synchrotron. The structure is clearly monomeric. The NMR structure of one domain was then translated and rotated relative to the other domain until a remarkably good fit to the distinctive SAXS data was obtained. The interaction between the two domains was then, and only then, inspected and found to involve a fairly loose and not implausible association via a small hydrophobic patch and several potential salt bridges. Residual dipolar coupling measurements are in progress to determine alignment vectors of the two domains, and confirm, or otherwise, the accuracy of the SAXS model of domain association. Cambiaghi TD, Pereira CM, Shanmugam R, Bolech M, Wek RC, Sattlegger E, Castilho BA. 2014. Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2. Biochem Biophys Res Commun 443: 592-597 (doi: 10.1016/j.bbrc.2013.12.021).
Keywords or phrases (comma separated) SAXS, structure modelling, protein NMR structure, solution state

Primary author

Prof. Geoffrey Jameson (Massey University)


Dr Evelyn Sattlegger (Massey University) Dr Gillian Norris (Massey University) Dr Goroncy Alexander (Massey University) Dr Harjes Elena (Massey University) Ms Natalie Burr (Massey University) Mr Trevor Loo (Massey University)

Presentation Materials

There are no materials yet.
Your browser is out of date!

Update your browser to view this website correctly. Update my browser now