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SUMMARY:Developing Bragg Coherent Diffractive Imaging for Biological cryst
 als at the Advanced Photon Source
DTSTART;VALUE=DATE-TIME:20141120T063000Z
DTEND;VALUE=DATE-TIME:20141120T080000Z
DTSTAMP;VALUE=DATE-TIME:20260510T104513Z
UID:indico-contribution-707@events01.synchrotron.org.au
DESCRIPTION:Speakers: Hannah Coughlan (La Trobe University)\nBragg Coheren
 t Diffractive Imaging (BCDI) of radiation hard crystals (e.g. lead\, gold)
  is now a relatively mature technique for characterising elastic strain fi
 elds at the nanoscale.  The technique relies upon the fact that coherent d
 iffraction from a crystal produces a continuous intensity distribution aro
 und every Bragg peak\; if sampled correctly\, this information can be used
  to reconstruct the crystals three-dimensional complex density. The corres
 ponding phase of the density is related to the elastic strain which provid
 es information on the atomic displacements within the crystal lattice (Pfe
 ifer et al\, 2006).\nFor protein crystals the presence of strain and disor
 der within the crystal can have a critical influence on the size of crysta
 l that can be grown and the quality of diffraction data that can be collec
 ted. Hence\, our group has recently been applying BCDI to the high-resolut
 ion characterisation of elastic strains in micron-sized protein crystals. 
 One of the major experimental challenges with such samples is that they ar
 e highly radiation sensitive\; however the combination of cryogenic temper
 atures and photon counting detectors offers a possible solution.\nHere we 
 present the first BCDI results collected from protein crystals of Lysozyme
  measuring ~3.5 microns in diameter using the newly developed cryo-BCDI se
 tup at beamline 34-ID-C at the Advanced Photon Source. Our preliminary rec
 onstructions from the BCDI data are promising\, and pave the way for strai
 n analysis of crystals composed of more complex proteins in future experim
 ents.  \nPfeifer\, M. et al(2006).Nature\,442(7098)\,63–6.\n\nhttps://ev
 ents01.synchrotron.org.au/event/3/contributions/707/
LOCATION: NCSS Exhibition Area
URL:https://events01.synchrotron.org.au/event/3/contributions/707/
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