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SUMMARY:In meso crystallization: Compatibility of Different Lipid Bicontin
 uous Cubic Mesophases with the Cubic Crystallization Screen in Aqueous Sol
 ution.
DTSTART;VALUE=DATE-TIME:20141120T221000Z
DTEND;VALUE=DATE-TIME:20141120T222000Z
DTSTAMP;VALUE=DATE-TIME:20260711T052334Z
UID:indico-contribution-702@events01.synchrotron.org.au
DESCRIPTION:Speakers: Leonie van 't Hag (University of Melbourne / CSIRO)\
 nA novel in meso crystallization method has facilitated the structural det
 ermination of several biologically relevant integral membrane proteins (IM
 Ps). However\, the method remains poorly understood as IMPs are difficult 
 to express and handle. Analogous to solution based crystallization\, in me
 so crystallization requires extensive screening of precipitant conditions.
  Bicontinuous cubic phases are the most commonly used lipid phases for in 
 meso crystallization. The compatibility of the crystallization screen used
  with the cubic phase is important\; if the underlying 3-D cubic nanostruc
 ture is destroyed\, the screen or protein and lipid combination may not be
  suitable for in meso crystallization experiments.\n\nWe looked at the imp
 act of a screen specifically marketed as compatible with the cubic mesopha
 se\, the Cubic crystallization screen (Emerald Biosystems)\, on the cubic 
 mesophases formed by three different lipids: monoolein\, monopalmitolein a
 nd phytantriol. The Cubic screen was found to be compatible with cubic mes
 ophase retention under most crystallization conditions studied. The effect
  of the individual components comprising the multicomponent screen was dec
 onvoluted in two ways. Initially\, the effect of specific poly(ethylene gl
 ycol) (PEG) and salt components on the cubic mesophase was determined usin
 g high-throughput synchrotron Small-Angle X-ray Scattering (SAXS). The eff
 ect of high-molecular-weight PEG was shown to dominate the phase behavior 
 within the screen. Finally\, a recently developed multiple linear regressi
 on modeling method was shown to deconvolute the effect of individual compo
 nents within the screen effectively.[1]\n\n[1] van ’t Hag\, L. et al.\, 
 Crystal Growth & Design\, 2014\, 14\, 1771-1781.\n\nhttps://events01.synch
 rotron.org.au/event/3/contributions/702/
LOCATION: Oliphant Auditorium
URL:https://events01.synchrotron.org.au/event/3/contributions/702/
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