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SUMMARY:Towards  real-time analysis of liquid jet alignment in SFX
DTSTART;VALUE=DATE-TIME:20211125T070700Z
DTEND;VALUE=DATE-TIME:20211125T070800Z
DTSTAMP;VALUE=DATE-TIME:20260306T090520Z
UID:indico-contribution-4226@events01.synchrotron.org.au
DESCRIPTION:Speakers: Jaydeep Jaydeep Patel (La Trobe University)\nSerial 
 femtosecond crystallography (SFX) enables atomic scale imaging of protein 
 structures via X-ray diffraction measurements from large numbers of small 
 crystals intersecting intense X-ray Free Electron Laser (XFEL) pulses. Sam
 ple injection typically involves continuous delivery of crystals to the pu
 lsed XFEL beam via a liquid jet. Due to movement of the jet\, which is oft
 en focused to further reduce its diameter using a gas virtual dynamic nozz
 le (GVDN)\, jet position is often adjusted multiple times during the exper
 iment. This can result in loss of beamtime and significant manual interven
 tion. Here we present a novel approach to the problem of liquid jet misali
 gnment in SFX based on machine vision. We demonstrate automatic identifica
 tion and classification of when there is overlap (‘hit’) and when ther
 e is not overlap (‘miss’) between the XFEL beam and jet. Our algorithm
  takes as its input optical images from the ‘side microscope’ located 
 inside the X-ray hutch. This algorithm will be incorporated into the contr
 ol system at the SFX/SPB beamline at the European XFEL where it will be us
 ed for in-situ ‘alignment correction’ via a continuous feedback loop w
 ith the stepper motors controlling the location of the nozzle within the c
 hamber. Full automation of this process will result in a larger volume of 
 useful data being collected. By increasing the efficiency and reducing the
  per experiment operational cost of SFX at the European XFEL a higher volu
 me of experiments can be performed. In addition\, via analysis of the feed
 back metrology we anticipate that optimised nozzle designs and jetting con
 ditions could be achieved further benefitting the end user.\n\nhttps://eve
 nts01.synchrotron.org.au/event/146/contributions/4226/
LOCATION:Online
URL:https://events01.synchrotron.org.au/event/146/contributions/4226/
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