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SUMMARY:Zeolitic imidazolate frameworks (ZIFs) structure and properties co
 rrelation to nucleic acid delivery
DTSTART;VALUE=DATE-TIME:20211125T065600Z
DTEND;VALUE=DATE-TIME:20211125T065700Z
DTSTAMP;VALUE=DATE-TIME:20260306T082659Z
UID:indico-contribution-4207@events01.synchrotron.org.au
DESCRIPTION:Speakers: Shakil Ahmed Polash (PhD candidate\, School of Scien
 ce\, RMIT University\, Melbourne\, Victoria 3000\, Australia)\nIn regenera
 tive medicine\, (intra)cellular delivery of genetic material can be used t
 o introduce functional copies of a gene that is defective and responsible 
 for disease development. To avoid nuclease- and lysosome-mediated degradat
 ion of the gene\, drug delivery systems / carriers need to be developed. R
 ecently\, non-viral delivery systems are being developed\, such as microin
 jection\, or various chemical approaches (e.g. liposomes\, polymers\, lipi
 ds)\; due to their economical synthesis\, biocompatibility and ability to 
 transfer a variety of genetic materials and gene editing tools.1 Zeolite i
 midazole framework (ZIF) is a well-studied non-viral polymeric delivery sy
 stem where coordination between Zn(II) and imidazolate forms a highly orga
 nised framework in aqueous solution. ZIFs offer advantageous physicochemic
 al properties for bio-delivery applications and have been shown to encapsu
 late a wide range of biomolecules\, including nucleic acids\, via biomimet
 ic mineralisation. Such ZIF-based delivery systems provide protection of t
 he gene cargo and were shown to result in endocytosis-mediated cellular up
 take. Further\, ZIFs degrade in the acidic microenvironments of cancer cel
 ls\, releasing their cargo at the target site.2\,3 Both cellular uptake an
 d release of ZIF encapsulated biomolecules are determined by the framework
  structure\, and its crystal phase. In our work\, a series of ZIF preparat
 ion methods are studied for the encapsulation of a circular plasmid. The r
 esulting ZIF structures are characterised via FTIR\, SEM\, synchrotron PXR
 D. The aim of this project is to establish structure–property relationsh
 ips to gene loading efficiency\, cellular uptake and cargo release profile
 s. \n\nReferences:\n1. Sung et al. 2019\, Biomater Res\, 23(1)\, 1-7\, doi
 : 10.1186/s40824-019-0156-z.\n2. Poddar et al. 2020\, Small\, 15(36)\, 190
 2268\, doi: 10.1002/smll.201902268.\n3. Poddar et al. 2021\, Chem Com\, 56
 (98)\,15406-15409\, doi: 10.1039/d0cc06241c.\n\nhttps://events01.synchrotr
 on.org.au/event/146/contributions/4207/
LOCATION:Online
URL:https://events01.synchrotron.org.au/event/146/contributions/4207/
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