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SUMMARY:Structural basis of the Trichoplax adhaerens Scribble and Dlg inte
 ractions with the PDZ-binding motif of Vangl
DTSTART;VALUE=DATE-TIME:20211125T064200Z
DTEND;VALUE=DATE-TIME:20211125T064300Z
DTSTAMP;VALUE=DATE-TIME:20260310T124146Z
UID:indico-contribution-4203@events01.synchrotron.org.au
DESCRIPTION:Speakers: Janesha Maddumage (1Department of Biochemistry and G
 enetics\, La Trobe University)\nMaintenance of multicellular tissue archit
 ecture is conserved process which is regulated by a highly\nconserved set 
 of proteins. The interacting partners of these regulators are also conserv
 ed across the\nanimal kingdom. Scribble and Dlg are two such key polarity 
 regulators that involve in the establishment\nand maintenance of multicell
 ular apical-basal cell polarity in epithelial cells. These are scaffolding
 \nproteins bearing multiple PDZ domains that mediate most of their interac
 tions. Complex multicellular\norganisms evolved from the simple primitive 
 forms\; therefore\, we examined Scribble and Dlg mediated\ncell polarity i
 n the simplest metazoan living on earth\, Placazoa\, Trichoplax adhaerens.
  Despite its\nextreme simplicity\, Trichoplax contains all polarity regula
 tors that are fundamental to instruct the body\nplans in higher animals\; 
 thus\, making it an ideal candidate to use as a polarity studying model. W
 e now\nshow biochemically that a key interaction for the establishment of 
 cell polarity between Scribble and\nDlg PDZ domains and Vangl in mammals i
 s fully recapitulated in Trichoplax. We found that Scribble\nPDZ1\, PDZ2 a
 nd PDZ3 interact with Vangl with affinities comparable to the human intera
 ction\, with\na similar hierarchy in affinities. We also found that all th
 ree PDZ domains of Dlg interact with Vangl\nwith no hierarchy of their aff
 inities. We then show using crystal structures of Scribble PDZ1\, PDZ2 and
 \nDlg PDZ1\, PDZ2 bound to the C-terminal PDZ binding motif of Vangl that 
 in addition to the binding\naffinities\, the detailed interactions between
  Scribble/Vangl and Dlg/Vangl are also conserved at the\natomic level betw
 een Trichoplax and human.\n\nhttps://events01.synchrotron.org.au/event/146
 /contributions/4203/
LOCATION:Online
URL:https://events01.synchrotron.org.au/event/146/contributions/4203/
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